| Name | PE Conf | Guide Box | Target RNA | Target Position | Match/Mismatch | Notes |
| sR1 | + | D' | 23S | G2008 | 11/0 | |
| D' | 23S | G2537 | 9/0 | |||
| D | 23S | C2582 | 11/0 | Non-CDP | ||
| sR2 | + | D' | 23S | U2529 | 9/1 | |
| D | 23S | C2649 | 13/0 | CDP | ||
| sR3 | + | D' | 23S | C2020 | 10/0 | CDP |
| D | 23S | G2049 | 10/0 | CDP | ||
| sR4 | + | D' | 23S | G1796 | 10/0 | |
| D' | 23S | G1341 | 10/0 | |||
| D | 23S | C2094 | 10/0 | Non-CPD | ||
| sR5 | + | D | 16S | C1209 | 11/0 | |
| D | 23S | C583 | 10/0 | |||
| sR6 | + | D' | 23S | C2034 | 12/0 | CDP |
| D | 23S | G1318 | 11/1 | |||
| sR7 | + | D' | 23S | U2591 | 11/0 | |
| D | 23S | G50 | 11/0 | CDP | ||
| sR8 | + | D' | 23S | G900 | 11/0 | |
| D' | tRNA Met | C34 | 10/0 |
PE Conf: the presence of the sRNA in total cellular RNA was verified
by primer extension. Guide Box: the position of the guide within
the sRNA (D or D' box associated). Target RNA: predicted target
of methylation. Target Position: predicted nucleotide postion of
methylation. Match/Mismatch: the number of Watson-Crick and G-U
matches / mismatches within the sRNA-target RNA duplex. Notes: "CDP",
dNTP concentration-dependent primer extension pause observed at the predicted
site of methylation, indicating a likely 2'-O-ribose methyl; "No Pause",
no pause was detected at either high or low dNTP concentrations. Guides
without notation were not experimentally examined.